What sequence is most likely to be recognized by a restriction enzyme?Ī recognition sequence of a restriction endonuclease must be a palindromic sequence. Restriction sites, or restriction recognition sites, are located on a DNA molecule containing specific (4-8 base pairs in length) sequences of nucleotides, which are recognized by restriction enzymes. Is restriction site and recognition site same? A recognition sequence is a specific sequence, usually very short (less than 10 bases). For example, there are two PstI recognition sites in the following DNA sequence fragment, starting at base 9 and 31 respectively. What do you mean by recognition site?Ī recognition site is specified by the position of the site. Today, scientists recognize three categories of restriction enzymes: type I, which recognize specific DNA sequences but make their cut at seemingly random sites that can be as far as 1,000 base pairs away from the recognition site type II, which recognize and cut directly within the recognition site and type III. Which type of restriction endonuclease cuts the DNA within the recognition site? Restriction enzymes, found naturally in bacteria, can be used to cut DNA fragments at specific sequences, while another enzyme, DNA ligase, can attach or rejoin DNA fragments with complementary ends. Typically, a restriction site will be a palindromic sequence of about four to six nucleotides long. A restriction enzyme is the site specific and makes cut specifically. The nucleotide sequence recognized for cleavage by a restriction enzyme is called as the restriction site. Which of the following DNA sequences could be the recognition site for a restriction enzyme? These regions are called recognition sequences, or recognition sites, and are randomly distributed throughout the DNA. What is the recognition site of restriction enzyme?Įach restriction enzyme recognizes a short, specific sequence of nucleotide bases (the four basic chemical subunits of the linear double-stranded DNA molecule-adenine, cytosine, thymine, and guanine). What does DNA ligase do to DNA fragments?.What would happen if the restriction enzymes do not cut the DNA at specific recognition sequences?.Is restriction site and recognition site same?.Which of the following DNA sequences could be the recognition site for a restriction enzyme?.What is the recognition site of restriction enzyme?.
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